Journal: International Journal of Medical Sciences
Article Title: CD44 mediates stem cell mobilization to damaged lung via its novel transcriptional targets, Cortactin and Survivin
doi: 10.7150/ijms.33125
Figure Lengend Snippet: CSC impairs migration and invasion ability of MSC mediated through CD44. (A) Gradual suppression of CD44 expression using CD44 SiRNA. CD44 inhibition was optimized by different doses of siRNA. Shown are the cropped blot images representing indicated proteins, and all the gels from three separate experiments have been run under the same experimental conditions. (B) Representative images of Boyden chamber membranes represent the number of captured cells during migration, illustrating the difference in migrated cell numbers. (C) Graphical representation of invading cells was demonstrated by invasion assay. Note a marked decrease in MSC cell migration and invasion capabilities after inhibiting CD44 expression using siRNA. Control cells were cultured in the same conditions. Representative images of Boyden chamber membranes represent the number of captured cells during the invasion, illustrating the difference in invaded cell numbers. The difference is considered statistically significant (student's t-test *P<0.001).
Article Snippet: Membranes were probed with mouse anti-CD44 (1:2000 dilution, R& D systems), mouse monoclonal anti CTTN antibody (1:2000 dilution, Upstate cell signaling solution), rabbit monoclonal anti BIRC5 antibody (1:2000 dilution, Santa Cruz Biotechnology)and goat anti-GAPDH antibody (1:2000 dilution, Santa Cruz, CA) and detected with a goat anti-mouse and donkey anti-goat IgG-HRP (1:5000 dilution, Santa Cruz, CA) using chemiluminescence (Supersignal West Femto, Pierce).
Techniques: Migration, Expressing, Inhibition, Invasion Assay, Cell Culture